Microscopy Imaging

Microscopy imaging techniques such as confocal and fluorescence microscopy permit visual and spatial investigation of fixed and live biological samples. A light source is used to excite a fluorophore on a target molecule that in turn emits light at a longer wavelength for detection.

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Nucleus Cores:

Leica DMI6000 Fluorescent Microscope

Capable of a variety of fluorescence microscopy and image analysis applications, including time lapse experiments. It has YFP, CFP, GFP, Texas Red, Cy5, and A4 filter cubes. It is equipped with the LAS and LAS AF software. Objectives include: Dry (2.5x, 10x, 20x and 40x) and Oil (40x and 60x/100x). The Leica DMI6000 has a colour camera in addition to the fluorescence camera that can be used for bright field, DIC, and phase contrast imaging.

Confocal Microscope

A Biorad 1024 MRC based on a Nikon Eclipse 800 microscope. This unit has the capability of combining double label imaging with DIC transmission optics. It has a krypton/argon laser with excitation at 488 nm, 568 nm and 647 nm. Objectives include 20x, 40x oil and 60x oil. The software includes quantitative analysis for distance staining intensity and double-label co-localization. Triple label sequential analysis is also available. A cryostat (Micron) is available for preparing sections for confocal imaging.

Leica Fluorescent Microscope

Contact Information

Principal Contact: Dr. Elizabeth Fidalgo

Institution: University of Windsor

Email: fidalgo@uwindsor.ca

Fees

User fee – $ 25.00/hour – annual charge for the total of hours used from April to March.

Cap fee – $ 1000.00 for 40 to 100 hrs and $ 2000.00 for more than 100 hrs per year.

Overnight time lapse imaging – $ 75.00 for an experiment.

Training fee – $ 25.00 per person – Training session is done during the first week of each month.

Confocal Microscope

Contact Information

Please contact Dr. Andrew Swan for training and user fee details

Principal Contact: Dr. Andrew Swan

Institution: University of Windsor

Email: aswan@uwindsor.ca

Microscopy Imaging

Leica DMI6000 Fluorescent Microscope

Confocal Microscope

Leica Fluorescent Microscope

Contact Information

Fees

Confocal Microscope

Contact Information

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